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to work with and they cost more than the plastic type. However, if your case has threaded holes in place of mounting slots, these brass hexagon spacers need to be used. The standoff has screw threads on one end and a threaded screw hole on the other end. The screw end is screwed into the case, and then the motherboard (along with some insulating Teflon, Delran, or paper washers) is attached to the other end with a screw. The washers are placed between the standoff and the motherboard and between the motherboard and the screw. This keeps the metal-edged mounting hole from contacting the screw and standoff and preventing it from shorting the board.

Reagents and Materials Sterile Reconstitution mixture, ice-cold (See Protocol 11.2) Complete medium (See Section 2.1) Petri dishes, 3.5 and 5 cm Non-sterile Silicone tubes (length: 10 mm, diameter: 2 mm) Te on disks (diameter: 16 mm, depth: 5 mm, central hole diameter: 2 mm) Te on cylinders (diameter: 8 mm, height: 10 mm) Liquid silicone glue Mechanical stretching device (See Fig. 11.4c, Color Plate 6B) Protocol (a) Glue two silicone tubes (length: 10 mm, diameter: 2 mm) to the surface of the 5-cm Petri dish to hold reversible Te on disks (diameter: 16 mm, depth: 5 mm, central hole diameter: 2 mm) serving as spacers while liquid silicone glue is poured into the culture dish. Te on spacers can be removed after hardening of the silicone glue to yield circular casting molds (See Fig. 11.4a, Color Plate 6B). (b) Leave casting molds for at least 2 weeks to ensure that silicone has thoroughly hardened and solvents have evaporated. (c) Wash molds and boil in distilled water before use. Subsequently, sterilize by autoclaving. Casting molds can be reused in nitely. (d) Before EHT casting, place removable Te on cylinders (diameter: 8 mm) over each silicone tubing to yield circular wells (750 mm3 ). The size of a casting mold is suf cient to hold the viscous reconstitution mixture (900 l) in place. (e) Pipette the ice-cold reconstitution mixture into the mold at room temperature. (f) Transfer the molds to the 37 C incubator for 1 hour. (g) Remove from the incubator, add 6 ml culture medium, and return to the incubator. (h) Culture EHTs in the casting mold for 7 days at 37 C, in a 5% CO2 -95% air, humidi ed atmosphere. (i) Change the medium after the initial overnight culture and then every other day.

(15.280)

Styles can contain font/character-formatting attributes and paragraph-formatting attributes. However, they cannot contain section-formatting attributes. Therefore, for example, you cannot create a style that would enable you to format a given selection with three columns and 1.5-inch left and right margins. Stand by for a few minutes, however, and you ll see how you can indeed effectively create a style for section formatting, although it s not really a style. Recall that in 6, Paragraph Formatting, you learned that the paragraph mark is the repository of paragraph formatting. Similarly, the section break is the repository of section formatting. If you delete a section break, the current section adopts the formatting of the section that follows, that is, the section whose section break is still intact. Where is the section break in a document that has only one section In fact, most documents have only a single section, so this is a serious and valid question. There is an implied section break at the end of the document, so if you insert a section break into a single-section document, the formatting for section 1 resides in that section break, and the formatting for section 2 effectively resides in the permanent paragraph mark at the end of the document. Permanent Yes, permanent. If you don t believe it, with paragraph marks displayed, delete everything in a document. Now delete that last paragraph mark. You can t do it! (You can hide it by clicking the Show/Hide button in the Paragraph group in the Home ribbon, but that s cheating, because it s not really gone.)